A laboratory-scale experiment was carried out to assess the influence of temperature fluctuation on thermophilic anaerobic digestion of municipal organic solid waste (MOSW). Heating failure was simulated by decreasing temperature suddenly from 55 °C to 20 °C suddenly; 2 h time is needed for temperature decrease and recovery. Under the conditions of 8.0 g/(L·d) and 15 d respectively for MOSW load and retention time, following results were noted: (1) biogas production almost stopped and VFA (volatile fatty acid) accumulated rapidly, accompanied by pH decrease; (2) with low temperature (20 °C) duration of 1, 5, 12 and 24 h, it took 3, 11, 56 and 72 h for the thermophilic anaerobic digestion system to reproduce methane after temperature fluctuation; (3) the longer the low temperature interval lasted, the more the methanogenic bacteria would decay; hydrolysis, acidification and methanogenesis were all influenced by temperature fluctuation; (4) the thermophilic microorganisms were highly resilient to temperature fluctuation.
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机译：进行了实验室规模的实验，以评估温度波动对城市有机固体废弃物（MOSW）的高温厌氧消化的影响。通过将温度从55°C突然降低到20°C来模拟加热失败；温度降低和恢复需要2小时的时间。在MOSW负荷和停留时间分别为8.0 g /（L·d）和15 d的条件下，注意到以下结果：（1）沼气生产几乎停止，VFA（挥发性脂肪酸）迅速积累，同时pH降低； （2）低温（20°C）持续时间为1、5、12和24小时，高温厌氧消化系统在温度波动后需要3、11、56和72小时来再生甲烷； （3）低温间隔持续的时间越长，产甲烷细菌的腐烂就越多；水解，酸化和甲烷生成均受温度波动的影响； （4）嗜热微生物对温度波动具有高度的适应力。
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