Effects of N6-benzylaminopurine and Indole Acetic Acid on In Vitro Shoot Multiplication Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa spp.)

摘要

Different concentrations of N⁶-benzylaminopurine (BAP) and indole acetic acid (IAA) in Murashige and Skoog based medium were assessed for their effects on shoot multiplication, nodule-like meristem proliferation and plant regeneration of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak. BAP at 1–14 mg L⁻¹ with or without 0.2 mg L⁻¹ IAA, or BAP at 7–14 mg L⁻¹ with the same concentration of IAA, was evaluated for shoot multiplication from shoot tips and the proliferation of nodule-like meristems from scalps, respectively. Plant regeneration from scalps was assessed using 1 mg L⁻¹ BAP and 0.2 mg L⁻¹ IAA separately, or a combination of these two growth regulators. Data on shoot multiplication, the proliferation of nodule-like meristems with associated plant regeneration were recorded after 30 days of culture. A maximum of 5 shoots per original shoot tip was achieved on medium supplemented with BAP at 5 mg L⁻¹ (Pisang Nangka), 6 mg L⁻¹ (Pisang Mas and Pisang Berangan), or 7 mg L⁻¹ (Pisang Awak), with 0.2 mg L⁻¹ IAA. BAP at 11 mg L⁻¹ with 0.2 mg L⁻¹ IAA induced the most highly proliferating nodule-like meristems in the four banana cultivars. Plant regeneration from scalps was optimum in all cases on medium containing 1 mg L⁻¹ BAP and 0.2 mg L⁻¹ IAA. This is the first report on the successful induction of highly proliferating nodule-like meristems and plant regeneration from scalps of the Malaysian banana cultivars Pisang Mas, Pisang Nangka, Pisang Berangan and Pisang Awak.