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Multi-Spectral Fluorescence Imaging of Colon Dysplasia InVivo Using a Multi-Spectral Endoscopy System

机译:使用多光谱内窥镜系统对结肠发育不良的多光谱荧光成像

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摘要

BACKGROUND AND STUDY AIM: To develop a molecular imaging endoscopic system that eliminates tissue autofluorescence and distinguishes multiple fluorescent markers specifically on the cancerous lesions. METHODS: Newly developed multi-spectral fluorescence endoscope device has the potential to eliminate signal interference due to autofluorescence and multiplex fluorophores in fluorescent probes. The multiplexing capability of the multi-spectral endoscope device was demonstrated in the phantom studies and multi-spectral imaging with endoscopy and macroscopy was performed to analyze fluorescence signals after administration of fluorescent probe that targets cancer in the colon. Because of the limitations in the clinical application using rigid-type small animal endoscope, we developed a flexible channel insert-type fluorescence endoscope, which was validated on the colonoscopy of dummy and porcine model. RESULTS: We measured multiple fluorescent signals simultaneously, and the fluorescence spectra were unmixed to separate the fluorescent signals of each probe, in which multiple fluorescent probes clearly revealed spectral deconvolution at the specific targeting area in the mouse colon. The positive area of fluorescence signal for each probe over the whole polyp was segmented with analyzing software, and showed distinctive patterns and significantly distinguishable values: 0.46 ± 0.04, 0.39 ± 0.08 and 0.73 ± 0.12 for HMRG, CET-553 and TRA-675 probes, respectively. The spectral unmixing was finally demonstrated in the dummy and porcine model, corroborating the targeted multi-spectral fluorescence imaging of colon dysplasia. CONCLUSION: The multi-spectral endoscopy system may allow endoscopists to clearly identify cancerous lesion that has different patterns of various target expression using multiple fluorescent probes.
机译:背景与研究目的:开发一种分子成像内窥镜系统,该系统可消除组织自体荧光并在癌性病变上专门区分多种荧光标记。方法:新开发的多光谱荧光内窥镜设备具有消除由于自发荧光和荧光探针中多重荧光团引起的信号干扰的潜力。在幻像研究中证明了多光谱内窥镜设备的复用能力,并且在施用靶向结肠癌的荧光探针后,通过内窥镜和宏观检查进行了多光谱成像以分析荧光信号。由于使用刚性小动物内窥镜的临床应用存在局限性,我们开发了一种柔性通道插入式荧光内窥镜,该模型已在假人和猪模型的结肠镜检查中得到验证。结果:我们同时测量了多个荧光信号,未混合荧光光谱以分离每个探针的荧光信号,其中多个荧光探针清楚地揭示了小鼠结肠特定靶向区域的光谱解卷积。用分析软件对每个探针在整个息肉上的荧光信号的阳性区域进行分割,并显示出独特的模式和明显可区别的值:HMRG,CET-553和TRA-675探针分别为0.46±0.04、0.39±0.08和0.73±0.12 , 分别。最后在假人和猪模型中证明了光谱的解混,证实了结肠发育不良的靶向多光谱荧光成像。结论:多光谱内窥镜系统可以使内镜医师使用多种荧光探针清楚地鉴定出具有不同靶标表达模式的癌灶。

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