Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing

摘要

IntroductionSuccessful stem cell therapy relies on large-scale generation of stem cells and their maintenance in a proliferative multipotent state. This study aimed to establish a three-dimension culture system for large-scale generation of hWJ-MSC and investigated the self-renewal activity, genomic stability and multi-lineage differentiation potential of such hWJ-MSC in enhancing skin wound healing.