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Complete genome sequence of Halomonas sp. R5-57

机译:Halomonas sp。的完整基因组序列。 R5-57

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摘要

The marine Arctic isolate Halomonas sp. R5-57 was sequenced as part of a bioprospecting project which aims to discover novel enzymes and organisms from low-temperature environments, with potential uses in biotechnological applications. Phenotypically, Halomonas sp. R5-57 exhibits high salt tolerance over a wide range of temperatures and has extra-cellular hydrolytic activities with several substrates, indicating it secretes enzymes which may function in high salinity conditions. Genome sequencing identified the genes involved in the biosynthesis of the osmoprotectant ectoine, which has applications in food processing and pharmacy, as well as those involved in production of polyhydroxyalkanoates, which can serve as precursors to bioplastics. The percentage identity of these biosynthetic genes from Halomonas sp. R5-57 and current production strains varies between 99 % for some to 69 % for others, thus it is plausible that R5-57 may have a different production capacity to currently used strains, or that in the case of PHAs, the properties of the final product may vary. Here we present the finished genome sequence () of Halomonas sp. R5-57 which will facilitate exploitation of this bacterium; either as a whole-cell production host, or by recombinant expression of its individual enzymes.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-016-0192-4) contains supplementary material, which is available to authorized users.
机译:海洋北极分离物Halomonas sp。 R5-57作为生物勘探项目的一部分进行了测序,该项目旨在从低温环境中发现新型酶和生物,并在生物技术应用中具有潜在用途。表型上,Halomonas sp。 R5-57在很宽的温度范围内均显示出较高的耐盐性,并具有与多种底物的细胞外水解活性,表明R5-57分泌可能在高盐度条件下起作用的酶。基因组测序确定了与渗透保护剂ectoine的生物合成有关的基因,该基因在食品加工和制药中都有应用,以及与聚羟基链烷酸酯的生产有关的基因,可以用作生物塑料的前体。这些来自Halomonas sp。的生物合成基因的百分比同一性。 R5-57和目前的生产菌株在某些产品的99%到其他产品的69%之间变化,因此,R5-57可能具有与当前使用的菌株不同的生产能力,或者在PHAs的情况下,最终产品可能会有所不同。在这里,我们介绍Halomonas sp。的完整基因组序列()。 R5-57,将促进该细菌的利用;电子补充材料本文的在线版本(doi:10.1186 / s40793-016-0192-4)包含补充材料,授权用户可以使用。

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