Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm2) and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase images indicate different conformation of the proteins and explain the cell selectivity.
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机译:通过氢和氧表面原子对金刚石表面进行微观化学构图,可用于将人类成骨细胞自组装为微阵列。细胞黏附和组装可通过细胞浓度(2,500-10,000个细胞/ cm 2 sup>)和胎牛血清(0-15%)来进一步控制。细胞通过肌动蛋白纤维和细胞核的荧光显微镜检查来表征。通过原子力显微镜(AFM)研究血清蛋白的吸附。单元以O端图案选择性地排列成30-200μm宽的阵列。较高的细胞浓度会由于相互之间的细胞通讯而使不利的H端区域定居。没有培养基中的蛋白质就没有细胞选择性。基于原子力显微镜,蛋白质同时存在于H和O末端表面。其厚度,表面粗糙度,形态和相图像的明显差异表明蛋白质的构象不同,并说明了细胞的选择性。
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