Diffusion Tensor Cardiac Magnetic Resonance Reveals Exosomes From Cardiosphere-Derived Cells Preserve Myocardial Fiber Architecture After Myocardial Infarction

摘要

class="kwd-title">Key Words: diffusion tensor MRI, exosomes, fiber architecture, regeneration, myocardial infarction class="kwd-title">Abbreviations and Acronyms: CDC, cardiosphere-derived cell; DT-CMR, diffusion tensor-cardiac magnetic resonance; EDV, end-diastolic volume; ESV, end-systolic volume; EF, ejection fraction; CDCEXO, exosomes secreted by CDC; HA, helix angle; HAT, helix angle transmurality; |HAT|, absolute helix angle transmurality; ICC, intra-class correlation; LGE, late gadolinium enhancement; LV, left ventricular; MI, myocardial infarction; MRI, magnetic resonance imaging; ROC, receiver operating characteristic; ROI, region of interest; SS, scar size; TD, transmural depth; TE, echo time; TR, repetition time class="head no_bottom_margin" id="abs0020title">SummaryThe object of the study was to reveal the fiber microstructural response with diffusion tensor cardiac magnetic resonance after intramyocardial exosomes secreted by cardiosphere-derived cells (CDCEXO) in chronic porcine myocardial infarction. Porcine with myocardial infarction underwent intramyocardial delivery of human CDCEXO and placebo in a randomized placebo-controlled study. Four weeks after injection, viability improved in the CDCEXO group, whereas myocardial fiber architecture and cardiac function were preserved. In the placebo group, fiber architecture and cardiac function declined. Myocardial regeneration by CDCEXO is not tumor-like; instead, details of tissue architecture are faithfully preserved, which may foster physiological excitation and contraction.