In vitro SCREENING ANTIBACTERIAL ACTIVITY OF Bidenspilosa LINNÉ AND Annona crassiflora MART. AGAINSTOXACILLIN RESISTANT Staphylococcus aureus (ORSA) FROM THE AERIALENVIRONMENT AT THE DENTAL CLINIC

摘要

Currently multiresistant Staphylococcus aureus is one common cause of infections with high rates of morbidity and mortality worldwide, which directs scientific endeavors in search for novel antimicrobials. In this study, nine extracts from Bidens pilosa (root, stem, flower and leaves) and Annona crassiflora (rind fruit, stem, leaves, seed and pulp) were obtained with ethanol: water (7:3, v/v) and their in vitro antibacterial activity evaluated through both the agar diffusion and broth microdilution methods against 60 Oxacillin Resistant S. aureus (ORSA) strains and against S. aureus ATCC6538. The extracts from B. pilosa and A. crassiflora inhibited the growth of the ORSA isolates in both methods. Leaves of B. pilosa presented mean of the inhibition zone diameters significantly higher than chlorexidine 0.12% against ORSA, and the extracts were more active against S. aureus ATCC (p < 0.05). Parallel, toxicity testing by using MTT method and phytochemical screening were assessed, and three extracts (B. pilosa, root and leaf, and A. crassiflora, seed) did not evidence toxicity. On the other hand, the cytotoxic concentrations(CC50 and CC90) for other extracts ranged from 2.06 to 10.77mg/mL. The presence of variable alkaloids, flavonoids, tannins and saponins wasobserved, even though there was a total absence of anthraquinones. Thus, the extractsfrom the leaves of B. pilosa revealed good anti-ORSA activity anddid not exhibit toxicity.