Differentiating inflamed and normal lungs by the apparent reaction rate constants of lactate dehydrogenase probed by hyperpolarized 13C labeled pyruvate

摘要

BackgroundClinically translatable hyperpolarized (HP) ¹³C-NMR can probe in vivo enzymatic reactions, e.g., lactate dehydrogenase (LDH)-catalyzed reaction by injecting HP ¹³C-pyruvate into the subject, which is converted to ¹³C labeled lactate by the enzyme. Parameters such as ¹³C-lactate signals and lactate-to-pyruvate signal ratio are commonly used for analyzing the HP ¹³C-NMR data. However, the biochemical/biological meaning of these parameters remains either unclear or dependent on experimental settings. It is preferable to quantify the reaction rate constants with a clearer physical meaning. Here we report the extraction of the kinetic parameters of the LDH reaction from HP ¹³C-NMR data and investigate if they can be potential predictors of lung inflammation.