Nuclear KATP channels trigger nuclearCa2+ transients that modulate nuclear function

摘要

Glucose, the principal regulator of endocrine pancreas, has several effects on pancreatic beta cells, including the regulation of insulin release, cell proliferation, apoptosis, differentiation, and gene expression. Although the sequence of events linking glycemia with insulin release is well described, the mechanism whereby glucose regulates nuclear function is still largely unknown. Here, we have shown that an ATP-sensitive K⁺ channel (KATP) with similar properties to that found on the plasma membrane is also present on the nuclear envelope of pancreatic beta cells. In isolated nuclei, blockade of the KATP channel with tolbutamide or diadenosine polyphosphates triggers nuclear Ca²⁺ transients and induces phosphorylation of the transcription factor cAMP response element binding protein. In whole cells, fluorescence in situ hybridization revealed that these Ca²⁺ signals may trigger c-myc expression. These results demonstrate a functional KATP channel in nuclei linking glucose metabolism, nuclear Ca²⁺signals, and nuclear function.