Increase in histidine decarboxylase activity in skin of genetically mast-cell-deficient W/Wv mice after application of phorbol 12-myristate 13-acetate: evidence for the presence of histamine-producing cells without basophilic granules.
Histidine decarboxylase (HisDCase, EC 4.1.1.22) activity in mouse skin increased by a factor of more than 10 after a single application of phorbol 12-myristate 13-acetate. The cell type that was responsible for the increase in HisDCase activity was examined by using (WB X C57BL/6)F1-W/Wv mice, which are genetically deficient in tissue mast cells. In contrast to a report that increase of ornithine decarboxylase (EC 4.1.1.17) activity occurs in the epidermis [O'Brien, T. G., Simisiman, R. C. & Boutwell, R. K. (1975) Cancer Res. 35, 2426-2433], the HisDCase activity was found to increase in the dermis. Although most of the histamine in the dermis was present in mast cells, the increase in HisDCase activity in the skin in W/Wv mice was comparable with that in congeneic +/+ mice. This increase of HisDCase activity in the skin of W/Wv mice was abolished by prior x-ray irradiation (800 rads; 1 rad = 0.01 gray) but was restored by subsequent bone marrow transplantation. Because mice, in general, are known to lack basophilic leukocytes, the present results suggest the existence of histamine-producing cells without basophilic granules that are derived from the bone marrow.
展开▼
机译：单次施用佛波醇12-肉豆蔻酸酯13-乙酸酯后，小鼠皮肤中的组氨酸脱羧酶（HisDCase，EC 4.1.1.22）活性增加了10倍以上。使用（WB X C57BL / 6）F1-W / Wv小鼠检查了负责HisDCase活性增加的细胞类型，这些小鼠在基因上缺乏组织肥大细胞。与报道鸟氨酸脱羧酶（EC 4.1.1.17）活性增加在表皮中发生的报道相反[O'Brien，T.G.，Simisiman，R.C。和Boutwell，R.K。（1975）Cancer Res。 35，2426-2433]，发现HisDCase活性在真皮中增加。尽管真皮中的大多数组胺都存在于肥大细胞中，但是W / Wv小鼠皮肤中HisDCase活性的增加与同类+ / +小鼠中的相似。 W / Wv小鼠皮肤中HisDCase活性的增加已通过先前的X射线照射（800 rads； 1 rad = 0.01 grey）消除，但随后的骨髓移植得以恢复。因为通常已知小鼠缺乏嗜碱性白细胞，所以目前的结果表明存在存在组胺的细胞，而没有衍生自骨髓的嗜碱性颗粒。
展开▼
