首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
【2h】

Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

机译:使用乙二醛和a啶橙分析聚丙烯酰胺和琼脂糖凝胶上的单链和双链核酸。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

We have developed a simple and rapid system for the denaturation of nucleic acids and their subsequent analysis by gel electrophoresis. RNA and DNA are denatured in 1 M glyoxal (ethanedial) and 50% (vol/vol) dimethyl sulfoxide, at 50 degrees. The glyoxalated nucleic acids are then subjected to electrophoresis through either acrylamide or agarose gels in a 10 mM sodium phosphate buffer at pH 7.0. When glyoxalated DNA molecules of known molecular weights are used as standards, accurate molecular weights for RNA are obtained. Furthermore, we have employed the metachromatic stain acridine orange for visualization of nucleic acids in gels. This dye interacts differently with double- and single-stranded polynucleotides, fluorescing green and red, respectively. By using these techniques, native and denatured DNA and RNA molecules can be analyzed on the same slab gel.
机译:我们已经开发了一种简单快速的系统,可用于核酸变性以及随后通过凝胶电泳进行的分析。 RNA和DNA在1 M乙二醛(乙二醛)和50%(体积/体积)的二甲基亚砜中于50度变性。然后将乙二醛化的核酸通过丙烯酰胺或琼脂糖凝胶在pH 7.0的10 mM磷酸钠缓冲液中进行电泳。当使用已知分子量的乙二醛化DNA分子作为标准品时,可以获得RNA的准确分子量。此外,我们已经使用了变色染料a啶橙来观察凝胶中的核酸。这种染料与双链和单链多核苷酸相互作用不同,分别发出绿色和红色荧光。通过使用这些技术,可以在同一平板凝胶上分析天然和变性的DNA和RNA分子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号