首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Plasminogen Activator Production Accompanies Loss of Anchorage Regulation in Transformation of Primary Rat Embryo Cells by Simian Virus 40
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Plasminogen Activator Production Accompanies Loss of Anchorage Regulation in Transformation of Primary Rat Embryo Cells by Simian Virus 40

机译:纤溶酶原激活物的产生伴随着猿猴病毒40转化原代大鼠胚胎细胞的锚定调节作用丧失。

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摘要

We have isolated several lines of rat embryo cells transformed by simian virus 40. All these lines are fully transformed with regard to saturation density and serum sensitivity, but they differ greatly in their anchorage dependence, as assayed by efficiency of plating in methyl cellulose suspension. This set of lines reveals a consistent relation of plasminogen activator production to plating efficiency in methyl cellulose. T-antigen-positive transformed lines that synthesize activator grow in methyl cellulose suspension, while T-antigen-positive transformed lines that do not synthesize activator fail to form colonies in suspension. Normal rat embryo cells produce very little plasminogen activator and do not grow in methyl cellulose. Sera that permit high levels of plasmin formation and activity support growth in semi-solid medium better than sera whose plasminogen is activated poorly and/or sera that contain inhibitors to plasmin.
机译:我们已经分离了几株由猿猴病毒40转化的大鼠胚胎细胞。就饱和密度和血清敏感性而言,所有这些细胞系均已完全转化,但通过在甲基纤维素悬浮液中进行铺板的效率分析,它们的锚定依赖性存在很大差异。这组线揭示了纤溶酶原激活剂的产生与甲基纤维素中电镀效率的一致关系。合成活化剂的T抗原阳性转化株在甲基纤维素悬浮液中生长,而未合成活化剂的T抗原阳性转化株不能在悬浮液中形成菌落。正常的大鼠胚胎细胞产生的纤溶酶原激活剂很少,并且不会在甲基纤维素中生长。允许高水平纤溶酶形成和活性的血清比其纤溶酶原激活不良的血清和/或含有纤溶酶抑制剂的血清更好地支持半固体培养基中的生长。

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