This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.
展开▼
机译：这项研究使用了基因替换策略来表征腺病毒（Ad）衣壳蛋白六邻体对血清型定义的贡献。通过用Ad2的序列替换Ad type 5（Ad5）六邻体基因，我们改变了嵌合病毒的类型特异性。决定型的表位主要与六邻体的环1相关，在较小程度上与环2相关。尽管嵌合六邻体病毒具有血清型独特性，但载体之间的表位相似性导致交叉水平较低。反应性中和抗体，与免疫系统的活化细胞和先天性武器相结合，足以抑制体内重新给药后的基因转导。
展开▼
