Immunodetection of the Ferredoxin-NADP+ Oxidoreductase-Binding Protein Complex in Thylakoids of Different Higher Plant Species

摘要

Monospecific polyclonal antibodies against thylakoid ferredoxin-NADP⁺ oxidoreductase and its binding protein from Spinacia oleracea were used to detect the presence of these proteins in different higher plants, including C3, C4, and Crassulacean acid metabolism species. A remarkable conservation of antigenic determinants in all the species analyzed was demonstrated for both the reductase and its binding protein. The association of these polypeptides in a complex was detected by immunoprecipitation.