Rational Design of Cyclic Antimicrobial Peptides Based on BPC194 and BPC198

摘要

A strategy for the design of antimicrobial cyclic peptides derived from the lead compounds c(KKLKKFKKLQ) (>BPC194) and c(KLKKKFKKLQ) (>BPC198) is reported. First, the secondary β-structure of >BPC194 and >BPC198 was analyzed by carrying out molecular dynamics (MD) simulations. Then, based on the sequence pattern and the β-structure of >BPC194 or >BPC198, fifteen analogues were designed and synthesized on solid-phase. The best peptides (>BPC490, >BPC918, and >BPC924) showed minimum inhibitory concentration (MIC) values <6.2 μM against Pseudomonas syringae pv. syringae and Xanthomonas axonopodis pv. vesicatoria, and an MIC value of 12.5 to 25 μM against Erwinia amylovora, being as active as >BPC194 and >BPC198. Interestingly, these three analogues followed the structural pattern defined from the MD simulations of the parent peptides. Thus, >BPC490 maintained the parallel alignment of the hydrophilic pairs K¹–K⁸, K²–K⁷, and K⁴–K⁵, whereas >BPC918 and >BPC924 included the two hydrophilic interactions K³–Q¹⁰ and K⁵–K⁸. In short, MD simulations have proved to be very useful for ascertaining the structural features of cyclic peptides that are crucial for their biological activity. Such approaches could be further employed for the development of new antibacterial cyclic peptides.