E- and Z-Isomers of New Phytoecdysteroid Conjugates from French Polynesian Microsorum membranifolium (Polypodiaceae) Fronds

摘要

Phytochemical investigation of the fronds of Microsorum membranifolium resulted in the isolation of a new phytoecdysteroid, E-2-deoxy-20-hydroxyecdysone 3-[4-(1-β-D-glucopyranosyl)]-caffeate (>1), together with two known phytoecdysteroids, E-2-deoxy-20-hydroxyecdysone 3-[4-(1-β-D-glucopyranosyl)]-ferulate (>2), E-2-deoxyecdysone 3-[4-(1-β-D-glucopyranosyl)]-ferulate (>3). Their respective Z-isomers >4–>6 were also observed and identified for the first time. The new structures were elucidated on the basis of extensive spectroscopic data analysis (1D, 2D-NMR and HR-MS techniques).