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Cyanobacterial Catalase Activity Prevents Oxidative Stress Induced by Pseudomonas fluorescens DUS1-27 from Inhibiting Brassica napus L. (canola) Growth

机译:蓝藻过氧化氢酶活性可防止荧光假单胞菌DUS1-27诱导的氧化胁迫抑制甘蓝型油菜的生长。

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摘要

Plant growth-promoting bacteria (PGPB) inhabit the rhizosphere of plants and are capable of enhancing plant growth through a number of mechanisms. A strain of Pseudomonas fluorescens DUS1-27 was identified as a potential PGPB candidate based on its ability to increase the growth of Brassica napus L. (canola) over that of uninoculated control plants in a soil-based system. The same P. fluorescens isolate was found to reduce plant growth in a hydroponic growth system, with plants showing the symptoms of a microbe-associated molecular pattern (MAMP) response to the bacteria. The amperometric quantification of H2O2, fluorescence-based total peroxidase assays, and quantification of catalase gene expression levels using qRT-PCR revealed that oxidative stress reduced plant growth in the hydroponic system. The addition of the cyanobacterium Nostoc punctiforme (known to have high catalase activity levels) in the hydroponic system as a co-inoculant reduced oxidative stress (49.7% decrease in H2O2 concentrations) triggered by the addition of P. fluorescens DUS1-27, thereby enabling plants to grow larger than uninoculated control plants. These results show the advantage of inoculating with multiple bacteria to promote plant growth and, for the first time, demonstrate that N. punctiforme beneficially assists plants under oxidative stress through its catalase activity in planta.
机译:促进植物生长的细菌(PGPB)居住在植物的根际中,并能够通过多种机制促进植物的生长。荧光假单胞菌DUS1-27菌株被鉴定为潜在的PGPB候选者,因为它具有比基于土壤的系统中未接种的对照植物增加甘蓝型油菜(canola)生长的能力。发现相同的荧光假单胞菌分离物减少了水培生长系统中的植物生长,其中植物表现出对细菌的微生物相关分子模式(MAMP)响应的症状。使用qRT-PCR对H2O2的安培定量,基于荧光的总过氧化物酶测定以及对过氧化氢酶基因表达水平的定量显示,氧化胁迫降低了水培系统中的植物生长。在水培系统中添加蓝藻Nostoc punctiforme(已知具有高的过氧化氢酶活性水平)作为辅菌减少了因添加荧光假单胞菌DUS1-27而引发的氧化应激(H2O2浓度降低了49.7%),从而使其能够植物长得比未接种的对照植物大。这些结果显示了接种多种细菌以促进植物生长的优势,并且首次证明点状猪笼草通过其在植物中的过氧化氢酶活性有益地协助植物在氧化胁迫下。

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