A simple and efficient CRISPR/Cas9 platform for induction of single and multiple heritable mutations in barley (Hordeum vulgare L.)

摘要

BackgroundGenome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimized for these types of plants. Here, we present the development of RNA-guided Cas9 system for simplex and multiplex genome editing in barley.