首页> 美国卫生研究院文献>The EMBO Journal >Resistance to the photosystem II herbicide diuron is dominant to sensitivity in the cyanobacterium Synechococcus sp. PCC7942
【2h】

Resistance to the photosystem II herbicide diuron is dominant to sensitivity in the cyanobacterium Synechococcus sp. PCC7942

机译:对光系统II除草剂杜隆的抗性在蓝细菌Synechococcus sp。的敏感性中占主导地位。 PCC7942

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

The transformable cyanobacterium, Synechococcus sp. PCC7942, was used to study the genetics of resistance to the herbicide diuron. In wild-type cells, diuron binds to one of the core proteins, called D1, of photosystem II reaction centres. This binding prevents the transfer of electrons from QA, the primary quinone acceptor, to QB, which is necessary to create the charge separation that drives ATP synthesis. A single amino acid substitution in the D1 protein reduces diuron binding and confers herbicide resistance to reaction centres containing the substituted D1 protein. In Synechococcus 7942, the D1 protein is encoded by three functional genes called psbAI, psbAII and psbAIII. By selectively altering one member at a time of the three-member psbA gene family, we have demonstrated that diuron-resistant alleles are dominant to diuron-sensitive alleles. The relative abundance of the different psbA gene transcripts is correlated with the fraction of diuron-resistant reaction centres and with the degree of diuron resistance. Growth in sublethal diuron selectively increases the steady-state levels of transcripts of genes (psbA and psbD) encoding the core proteins of photosystem II. We have also found that turnover of the D1 protein can be uncoupled from electron transport through photosystem II.
机译:可转化的蓝细菌Synechococcus sp。 PCC7942用于研究对除草剂杜隆的抗性遗传。在野生型细胞中,敌草隆与光系统II反应中心的一种核心蛋白D1结合。这种结合可以防止电子从主要的醌接受体QA转移到QB,而这是产生驱动ATP合成的电荷分离所必需的。 D1蛋白中的单个氨基酸取代减少了diuron结合,并使除草剂对包含取代的D1蛋白的反应中心具有抗性。在Synechococcus 7942中,D1蛋白由三个功能基因psbAI,psbAII和psbAIII编码。通过有选择地一次更改三个成员的psbA基因家族中的一个成员,我们已经证明,耐草隆的等位基因对草隆敏感的等位基因占优势。不同的psbA基因转录本的相对丰度与耐diuron反应中心的分数和耐diuron程度相关。亚致死性diuron的生长选择性地增加了编码光系统II核心蛋白的基因(psbA和psbD)的转录本的稳态水平。我们还发现,D1蛋白的更新可以与通过光系统II的电子传输解耦。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号