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The use of alkalinity and incubation at 9 degrees C for improved recovery of Yersinia spp. from faeces.

机译:使用碱度和在9摄氏度下孵育可提高耶尔森菌的回收率。从粪便。

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摘要

Recovery of Yersinia enterocolitica and related strains from faecal samples enriched in 1% buffered peptone water (pH 7.2) and incubated at 4 degrees C for 7-21 days was compared with recovery from 1% peptone water buffered to pH 8.0 and incubated over the temperature range 4-26 degrees C. Best recovery was obtained by use of the alkaline medium incubated at 9 degrees C. Greatest recovery was obtained after incubation for 10-14 days, but most strains (greater than 75%) were recovered after 1 week.
机译:从富含1%缓冲蛋白ept水(pH 7.2)并在4°C下孵育7-21天的粪便样品中回收小肠结肠炎耶尔森氏菌和相关菌株,并与从缓冲至pH 8.0并在此温度下孵育的1%蛋白ept水的回收率进行比较温度范围为4-26摄氏度。使用在9摄氏度下孵育的碱性培养基可获得最佳的回收率。孵育10-14天后可获得最大的回收率,但是大多数菌株(大于75%)在1周后得以回收。

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