首页> 美国卫生研究院文献>EvoDevo >Ontogenetic differences in localization of glutamine transporter ApGLNT1 in the pea aphid demonstrate that mechanisms of host/symbiont integration are not similar in the maternal versus embryonic bacteriome
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Ontogenetic differences in localization of glutamine transporter ApGLNT1 in the pea aphid demonstrate that mechanisms of host/symbiont integration are not similar in the maternal versus embryonic bacteriome

机译:豌豆蚜中谷氨酰胺转运蛋白ApGLNT1定位的个体发育差异表明宿主/共生体整合的机制在母体和胚胎细菌组中并不相似

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摘要

BackgroundObligate intracellular symbionts of insects are metabolically and developmentally integrated with their hosts. Typically, reproduction fails in many insect nutritional endosymbioses when host insects are cured of their bacterial symbionts, and yet remarkably little is known about the processes that developmentally integrate host and symbiont. Here in the best studied insect obligate intracellular symbiosis, that of the pea aphid, Acyrthosiphon pisum, with the gammaproteobacterium Buchnera aphidicola, we tracked the expression and localization of amino acid transporter ApGLNT1 gene products during asexual embryogenesis. Recently being characterized as a glutamine transporter, ApGLNT1 has been proposed to be a key regulator of amino acid biosynthesis in A. pisum bacteriocytes. To determine when this important mediator of the symbiosis becomes expressed in aphid embryonic bacteriocytes, we applied whole-mount in situ hybridization and fluorescent immunostaining with a specific anti-ApGLNT1 antibody to detect the temporal and spatial expression of ApGLNT1 gene products during asexual embryogenesis.
机译:背景昆虫的专性细胞内共生体与其宿主代谢和发育整合。通常,当宿主昆虫治愈细菌共生体时,在许多昆虫营养内共生体中繁殖均会失败,但对于发育整合宿主和共生体的过程知之甚少。在最深入研究的昆虫专一性细胞内共生中,豌豆蚜虫,豌豆蚜虫与γ-变形杆菌Buchnera aphidicola进行了共生,我们追踪了无性胚胎发生过程中氨基酸转运蛋白ApGLNT1基因产物的表达和定位。最近被认为是谷氨酰胺转运蛋白的ApGLNT1被认为是Pisum细菌细胞中氨基酸生物合成的关键调节剂。为了确定这种重要的共生介质何时在蚜虫的胚胎细菌细胞中表达,我们应用了原位杂交和荧光免疫染色与特定的抗ApGLNT1抗体,以检测无性胚胎发生过程中ApGLNT1基因产物的时空表达。

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