Budding Yeast SLX4 Contributes to the Appropriate Distribution of Crossovers and Meiotic Double-Strand Break Formation on Bivalents During Meiosis

摘要

The number and distribution of meiosis crossover (CO) events on each bivalent are strictly controlled by multiple mechanisms to assure proper chromosome segregation during the first meiotic division. In Saccharomyces cerevisiae, is a multi-functional scaffold protein for structure-selective endonucleases, such as and (which are involved in DNA damage repair), and is also a negative regulator of the -dependent signaling pathway with . has been believed to play only a minor role in meiotic recombination. Here, we report that is involved in proper intrachromosomal distribution of meiotic CO formation, especially in regions near centromeres. We observed an increase in uncontrolled CO formation only in a region near the centromere in the ∆ mutant. Interestingly, this phenomenon was not observed in the ∆, ∆, or ∆ mutants. In addition, we observed a reduced number of DNA double-strand breaks (DSBs) and altered meiotic DSB distribution on chromosomes in the ∆ mutant. This suggests that the multi-functional is required for proper CO formation and meiotic DSB formation.