A search for doxycycline-dependent mutations that increase Drosophila melanogaster life span identifies the VhaSFD Sugar baby filamin fwd and Cctl genes

摘要

BackgroundA P-type transposable element called PdL has been engineered with a doxycycline-inducible promoter directed out through the 3' end of the element. Insertion of PdL near the 5' end of a gene often yields doxycycline-dependent overexpression of that gene and a mutant phenotype. This functional genomics strategy allows for efficient screening of large numbers of genes for overexpression phenotypes.