Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for insulation but binding of TFIIIB along with TFIIIC likely improves the probability of complex formation at an insulator. Insulation by this transcription factor occurs in the absence of RNA polymerase III or polymerase II but requires specific histone acetylases and chromatin remodelers. This analysis identifies a minimal set of factors required for insulation.
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机译:染色质绝缘子将活性区和抑制的染色质区分开。在酵母中,与tRNA基因结合的RNA pol III转录机制与组蛋白乙酰酶和染色质重塑剂一起起作用,以限制异染色质的扩散。我们的结果共同证明,TFIIIC的结合对于绝缘是必需的,但是TFIIIB与TFIIIC的结合可能会提高在绝缘子上形成复合物的可能性。这种转录因子的绝缘作用是在不存在RNA聚合酶III或聚合酶II的情况下发生的,但需要特定的组蛋白乙酰化酶和染色质重塑剂。该分析确定了绝缘所需的最少因素集。
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