Three-year monitoring of genetic diversity reveals a micro-connectivity pattern and local recruitment in the broadcast marine species Paracentrotus lividus

摘要

In conservation and management of marine biological resources, a knowledge of connectivity is necessary to understand how local populations are naturally replenished by the arrival of new recruits from source populations. At small geographical scales, species experiencing moderate to long pelagic larval phases are mostly genetically homogeneous, which hinders inferences about local connectivity. Recent studies demonstrated that assessing genetic relatedness and kinship could provide information about local connectivity in populations with high levels of gene flow. Here, we were interested in deciphering the structure and connectivity of populations of the sea urchin Paracentrotus lividus, by monitoring populations at 11 localities distributed along a 225-km coast-line in the south-eastern French Mediterranean Sea. Using 12 microsatellite loci, we found a weak but significant genetic differentiation and observed a transient genetic differentiation among locations within temporal cohorts, without any correlation with the distance between locations, interpreted as unexplainable chaotic genetic patchiness. Among temporal cohorts, the more related individuals were mainly found within locations and the observed local differentiation (FST) correlated with the proportion of kin within locations, suggesting that larvae dispersed cohesively. Specifically, we could also reveal that populations flanking Cape Sicié were influenced by eastern populations and that local recruitment was a frequent occurrence. Overall, our results contribute to the growing number of studies showing that connectivity can be reliably assessed at a fine spatial scale even in genetically homogenous populations.