Effects of Ozone Oxidative Preconditioning on TNF-α Release and Antioxidant-Prooxidant Intracellular Balance in Mice During Endotoxic Shock

摘要

Ozone oxidative preconditioning is a prophylactic approach, which favors the antioxidant-prooxidant balance for preservation of cell redox state by the increase of antioxidant endogenous systems in both in vivo and in vitro experimental models. Our aim is to analyze the effect of ozone oxidative preconditioning on serum TNF-α levels and as a modulator of oxidative stress on hepatic tissue in endotoxic shock model (mice treated with lipopolysaccharide (LPS)). Ozone/oxygen gaseous mixture which was administered intraperitoneally (0.2, 0.4, and 1.2 mg/kg) once daily for five days before LPS (0.1 mg/kg, intraperitoneal). TNF-α was measured by cytotoxicity on L-929 cells. Biochemical parameters such as thiobarbituric acid reactive substances (TBARS), enzymatic activity of catalase, glutathione peroxidase, and glutathione-S transferase were measured in hepatic tissue. One hour after LPS injection there was a significant increase in TNF-α levels in mouse serum. Ozone/oxygen gaseous mixture reduced serum TNF-α levels ina dose-dependent manner. Statistically significant decreases inTNF-α levels after LPS injection were observed in micepretreated with ozone intraperitoneal applications at 0.2(78%), 0.4 (98%), and 1.2 (99%). Also a significantincrease in TBARS content was observed in the hepatic tissue ofLPS-treated mice, whereas enzymatic activity of glutathion-Stransferase and glutathione peroxidase was decreased. However inozone-treated animals a significant decrease in TBARS content wasappreciated as well as an increase in the activity of antioxidantenzymes. These results indicate that ozone oxidativepreconditioning exerts inhibitory effects on TNF-αproduction and on the other hand it exerts influence on theantioxidant-prooxidant balance for preservation of cell redoxstate by the increase of endogenous antioxidant systems.