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Extracellular Ligninolytic Enzymes in Bjerkandera adusta and Lentinus squarrosulus

机译:Bjerkandera裂纹和LENTINUS squarrosulus中的细胞外木质素分解酶

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摘要

Extracellular ligninolytic enzyme activities were determined in two white-rot fungi, Bjerkandera adusta and Lentinus squarrosulus. To investigate the activity of extracellular enzymes, cultures were incubated over a period of 20 days in nutrient rich medium (NRM) and nutrient poor medium under static and shaking conditions. Enzymatic activity was varied with media and their incubation conditions. The highest level of Aryl alcohol oxidase (AAO) was detected under shaking condition of both medium while Manganese peroxidase (MnP) activity was best in NRM under both conditions. AAO is the main oxidases enzyme in B. adusta while laccase plays important role in L. squarrosulus. MnP is the main peroxidase enzyme in both varieties.
机译:测定了两种腐烂真菌Bjerkandera adusta和Lentinus squarrosulus的细胞外木质素分解酶活性。为了研究细胞外酶的活性,在静态和摇动条件下,将培养物在富含营养的培养基(NRM)和缺乏营养的培养基中孵育20天。酶活性随培养基及其孵育条件而变化。在两种培养基的摇动条件下均检测到最高水平的芳醇氧化酶(AAO),而在两种条件下,NRM中锰过氧化物酶(MnP)活性均最佳。 AAO是B. adusta中的主要氧化酶,而漆酶在L. squarrosulus中起重要作用。在这两个品种中,MnP是主要的过氧化物酶。

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