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>Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle.
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Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle.
The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge.
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机译：磷脂酶D（PLD）基因（pld）已通过使用位点特异性诱变从假结核杆菌染色体中删除。绵羊感染试验表明，PLD阴性假结核梭菌菌株（Toxminus）即使在比野生型菌株产生该病的剂量高两个对数的剂量下，也无法诱导干酪状淋巴结炎（干酪腺）。这显然将PLD确立为主要的假结核梭菌毒力因子。用活的Toxminus C. pseudotuberculosis接种绵羊会引起强烈的体液和细胞介导的免疫反应，并保护动物免受野生型攻击。
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