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Use of Monoclonal Antibody in Diagnosis of Candidiasis Caused by Candida albicans: Detection of Circulating Aspartyl Proteinase Antigen

机译:单克隆抗体在诊断中的应用 白色念珠菌引起的念珠菌病:检测 循环天冬氨酰蛋白酶抗原

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摘要

To develop a serological diagnosis of invasive candidiasis based on detection of circulating secreted aspartyl proteinase (SAP) antigen of Candida albicans, three different enzyme-linked immunosorbent assays (ELISAs) were compared. The first was a standard ELISA to detect anti-SAP antibodies, and the others were an antigen capture ELISA and an inhibition ELISA to detect circulating SAP antigen with monoclonal antibody (MAb) CAP1, which is highly specific for SAP. These tests were applied to 33 serum samples retrospectively selected from 33 patients with mycologically and/or serologically proven invasive candidiasis caused by C. albicans. Serum samples from 12 patients with aspergillosis and serum samples from 13 healthy individuals were also included. The sensitivities and specificities were 69.7 and 76.0% for the standard ELISA and 93.9 and 92.0% for the antigen capture ELISA, respectively. However, these values reached 93.9 and 96.0%, respectively, for the inhibition ELISA. Serum samples from 31 of 33 patients had detectable SAP antigen, with concentrations ranging from 6.3 to 19.0 ng/ml. These results indicate that the inhibition ELISA with MAb CAP1 is effective in detection of circulating SAP antigen and that this assay may be useful for diagnosis and treatment monitoring of invasive candidiasis.
机译:为了通过检测白色念珠菌的循环分泌天冬氨酰蛋白酶(SAP)抗原来开发侵袭性念珠菌病的血清学诊断,比较了三种不同的酶联免疫吸附测定(ELISA)。第一种是用于检测抗SAP抗体的标准ELISA,另一种是用于通过SAP高度特异性的单克隆抗体(MAb)CAP1检测抗原的ELISA和抑制ELISA,以检测循环中的SAP抗原。这些测试应用于33份血清样本,这些样本是从33例经白色念珠菌引起的经真菌学和/或血清学证实的浸润性念珠菌病患者中回顾性选择的。还包括了12例曲霉病患者的血清样本和13例健康个体的血清样本。标准ELISA的敏感性和特异性分别为69.7%和76.0%,抗原捕获ELISA的敏感性和特异性分别为93.9和92.0%。然而,对于抑制ELISA,这些值分别达到93.9%和96.0%。 33名患者中有31名患者的血清样本具有可检测的SAP抗原,浓度范围为6.3至19.0 ng / ml。这些结果表明 MAb CAP1抑制酶联免疫吸附测定可有效检测血液循环 SAP抗原,该测定法可能对诊断和 侵袭性念珠菌病的治疗监测。

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