Zfp422 promotes skeletal muscle differentiation by regulating EphA7 to induce appropriate myoblast apoptosis

摘要

Zfp422 is essential for developmental myogenesis and primary myoblast differentiation. Quantitative PCR for the expression of Zfp422, Myogenin and MyHC in the whole embryo (E9) and dorsal muscle (E11-P56) at the indicated ages. Data are presented as mean  = 3 per group ( ). Staining for Zfp422 and nuclei (DAPI) on the dorsal muscle cross section of WT E17. Co-immunostaining for Zfp422 with markers of progenitors (Pax7), myoblasts (MyoD) and myofibers (MyHC) on dorsal muscle cross sections of WT E17. Zfp422 protein levels in the E17 dorsal muscle of Zfp422+/+;Myf5Cre/+(control) and Zfp422fl/fl;Myf5Cre/+(Zfp422-mKO) embryos. Weight of control and Zfp422-mKO embryos at E17. Data are presented as mean ± SD. (control) = 9, (Zfp422-mKO) = 8. Staining for MyHC and nuclei (DAPI) on E17 dorsal muscle cross sections. Mean myofiber CSA and the number of myofibers per 10,000 μm were counted. mRNA expression of indicated genes in control and Zfp422-mKO E17 mouse dorsal muscle. Myoblasts isolated from Zfp422-mKO E17 embryos and control were differentiated for 3 days, and stained for MyHC and nuclei. The differentiation index and fusion index were counted. Scale bars represent 50 μm in , , and 100 μm in . **  p