1 Very highly purified (greater than 100,000 u/mg) slow reacting substance of anaphylaxis (SRS-A) has been prepared by reversed phase high pressure liquid chromatographic (HPLC) techniques. 2 High resolution liquid chromatography suggests that SRS-A may exist in at least three distinct forms which are possible tautomeric. 3 SRS-A collected by antigen challenge in vivo and by calcium ionophore-induced release in vitro are chromatographically indistinguishable. 4 Treatment of SRS-A with diazomethane but not sodium borohydride results in a loss of biological activity but treatment of the methyl ester with base results in a partial recovery of activity. 5 Highly purified SRS-A was examined by infrared and ultra-violet spectroscopy, and found to have a benzene-aromatic and probably an amino acid.
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机译：1已通过反相高压液相色谱（HPLC）技术制备了非常高纯度（大于100,000 u / mg）的过敏反应慢反应物质（SRS-A）。 2高分辨率液相色谱表明，SRS-A可能以可能互变异构的至少三种不同形式存在。通过体内抗原攻击和体外钙离子载体诱导释放收集的3 SRS-A在色谱上无法区分。 4用重氮甲烷而不是硼氢化钠处理SRS-A会导致生物活性降低，但是用碱处理甲酯会导致部分活性恢复。 5通过红外和紫外光谱法检查了高度纯化的SRS-A，发现其具有苯芳香族化合物，可能还有氨基酸。
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