The neuroprotection induced by Hypericum brasiliense Choisy extract (HBE) and its main active polyphenol compound quercetin, against Crotalus durissus terrificus (Cdt) venom and crotoxin and crotamine, was enquired at both central and peripheral mammal nervous system. Cdt venom (10 μg/mL) or crotoxin (1 μg/mL) incubated at mouse phrenic nerve-diaphragm preparation (PND) induced an irreversible and complete neuromuscular blockade, respectively. Crotamine (1 μg/mL) only induced an increase of muscle strength at PND preparations. At mouse brain slices, Cdt venom (1, 5, and 10 μg/mL) decreased cell viability. HBE (100 μg/mL) inhibited significantly the facilitatory action of crotamine (1 μg/mL) and was partially active against the neuromuscular blockade of crotoxin (1 μg/mL) (data not shown). Quercetin (10 μg/mL) mimicked the neuromuscular protection of HBE (100 μg/mL), by inhibiting almost completely the neurotoxic effect induced by crotoxin (1 μg/mL) and crotamine (1 μg/mL). HBE (100 μg/mL) and quercetin (10 μg/mL) also increased cell viability in mice brain slices. Quercetin (10 μg/mL) was more effective than HBE (100 μg/mL) in counteracting the cell lysis induced by Cdt venom (1 and 10 μg/mL, resp.). These results and a further phytochemical and toxicological investigations could open new perspectives towards therapeutic use of Hypericum brasiliense standardized extract and quercetin, especially to counteract the neurotoxic effect induced by snake neurotoxic venoms.
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机译：在哺乳动物的中枢神经系统和外周神经系统中，都询问了巴西贯叶连翘提取物（HBE）及其主要活性多酚类化合物槲皮素对猪屎豆（Ctalt durissus terrificus，Cdt）毒液，crotoxin和crotamine的神经保护作用。在小鼠神经-制备物中（PND）孵育的Cdt毒液（10μg/ mL）或crotoxin（1μg/ mL）分别引起了不可逆的和完全的神经肌肉阻滞。在PND制剂中，巴豆胺（1μg/ mL）仅引起肌肉力量增加。在小鼠脑切片上，Cdt毒液（1、5和10μg/ mL）降低了细胞活力。 HBE（100μg/ mL）显着抑制了巴豆胺（1μg/ mL）的促进作用，并部分抵抗crotoxin的神经肌肉阻滞（1μg/ mL）（数据未显示）。槲皮素（10 μ g / mL）模仿了HBE的神经肌肉保护作用（100 μ g / mL），通过几乎完全抑制克罗毒素（1 μ g / mL）和巴豆胺（1 μ ）诱导的神经毒性作用g / mL）。 HBE（100 μ g / mL）和槲皮素（10 μ g / mL）还可增加细胞活力小鼠脑片。槲皮素（10 μ g / mL）比HBE（100 μ g / mL）更有效Cdt毒液诱导的细胞裂解（分别为1和10 μ g / mL）。这些结果以及进一步的植物化学和毒理学研究可以为治疗性 Hypericum brasiliense 标准化提取物和槲皮素开辟新的视角，尤其是抵消蛇神经毒性毒液引起的神经毒性作用。
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