Experimental Evaluation and Optimization of Structuresfor Lossless Ion Manipulations for Ion Mobility Spectrometry withTime-of-Flight Mass Spectrometry

摘要

We report on the performance of structures for lossless ion manipulation (SLIM) as a means for transmitting ions and performing ion mobility separations (IMS). Ions were successfully transferred from an electrospray ionization (ESI) source to the TOF MS analyzer by means of a linear SLIM, demonstrating lossless ion transmission and an alternative arrangement including a 90° turn. First, the linear geometry was optimized for radial confinement by tuning RF on the central “rung” electrodes and potentials on the DC-only guard electrodes. Selecting an appropriate DC guard bias (2–6 V) and RF amplitude (≥160 Vp-p at 750 kHz) resulted in the greatest ion intensities. Close to ideal IMS resolving power was maintained over a significant range of applied voltages. Second, the 90° turn was optimized for radial confinement by tuning RF on the rung electrodes and DC on the guard electrodes. However, both resolving power and ion transmission showed a dependence on these voltages, and the best conditions for both were >300 Vp-p RF (685 kHz) and 7–11V guard DC bias. Both geometries provide IMS resolving powers at thetheoretical limit (R ∼ 58), showing that degradedresolution from a “racetrack” effect from turning arounda corner can be successfully avoided, and the capability also wasmaintained for essentially lossless ion transmission.