化痰行瘀汤对肝纤维化大鼠组织病理学及羟脯氨酸含量的影响

摘要

Objective To assess the therapeutic effects of Huatan Xingyu (HTXY) decoction on experimental hepatic fi-brosis in rats. Methods Sixty adult Wistar rats were included into 6 groups (n=10 in each):normal control group, model control group, Fufang Biejia Ruangan group, low- dose HTXY group, middle- dose HTXY group and high- dose HTXY group. The corre-sponding drugs were given for 6 weeks. After treatment, the hydroxyproline(Hyp) levels in hepatic tissues were detected by alkali hydrolysis method and the pathological changes of hepatic fibrosis were observed by HE staining and picric acid- sirius red staining. Results Compared to normal control and model control groups, the degree of hepatic fibrosis were significantly atten-uated in Fufang biejia ruangan group and HTXY decoction groups (P<0.05), and more markedly in middle- dose group. The Hyp levels in hepatic tissues were also significantly decreased in middle- dose HTXY group (P<0.05). Conclusion HTXY decoction can improve hepatic fibrosis and reduce hepatic tissue Hyp levels in rats with experimental hepatic fibrosis.%目的观察化痰行瘀汤对肝纤维化的疗效，并进一步探讨其抗纤维化的作用机制。方法取清洁级健康成年Wis-tar大鼠60只，随机抽取10只作为正常对照组，其余50只采用经典模型复制后随机数字表法分为5组（造模过程中死亡11只），模型对照组（10只）、复方鳖甲软肝片组（7只）、和化痰行瘀汤低（7只）、中（7只）、高（6只）剂量组，分别以相应药物连续干预6周。采用样本碱水解法检测各组大鼠部分肝组织羟脯氨酸（Hyp）含量；并采用常规HE染色和苦味酸-天狼红染色观察肝纤维化程度。结果复方鳖甲软肝片组、化痰行瘀汤各剂量组能明显改善肝纤维化程度；化痰行瘀汤中剂量组的病理改变明显，明显降低肝组织Hyp含量（P＜0.05）。结论化痰行瘀汤能降低肝组织Hyp含量，明显减轻病理改变，有效改善肝纤维化。