胃癌组织中水通道蛋白1、微血管密度与细胞胀亡的关系及临床意义

摘要

Objective To investigate the relationship of aquaporin- 1 (AQP1) and microvessel density (MVD) with the on-cosis in gastric carcinoma and its clinical significance. Methods Expression of AQP1 in 55 cases of gastric cancer and 22 cas-es of normal gastric tissue was examined by immunohistochemical method and Western blot. MVD was examined by immunohis-tochemical method. Oncosis was observed by transmission electron microscope. Both the expression of AQP1 and MVD were measured by the image analysis.Al 55 patients were fol owed up and the relationship of AQP1 and MVD with patients' survival was analyzed. Results Oncosis was observed by transmission electron microscopy in tissue specimens of gastric carcinoma. The oncosis index (OI) was decreased with decreasing of clinical staging (P<0.01) and it was higher in cases with lymph node metastasis than those without (P<0.05). The expression of AQP1 was positively correlated with MVD (P<0.05). In gastric cancer tissue, the positive expression rate of AQP1 was 58.2%, significantly higher than that in the normal gastric tissue (P<0.01). AQP1 was significantly correlated with lymphatic metastasis. Multivariate analysis revealed that AQP1 expression and lymph node metastasis were independent prognostic factors of poor survival (P<0.05). Conclusion AQP1 may contribute to the progression of gastric cancer by inhabiting oncosis and leading to angiogenesis, and it can be used as an independent prognostic factor for gastric carcinoma.%目的：探讨胃癌组织中水通道蛋白1（AQP1）、微血管密度（MVD）与细胞胀亡的关系及临床意义。方法采用免疫组化法、蛋白印迹法检测55例胃癌、22例胃正常组织中AQP1的表达，Ⅷ因子相关抗原多克隆抗体标记血管内皮细胞法计数MVD，透射电镜观测细胞胀亡情况。随访并分析AQP1与胃癌患者预后的关系。结果胃癌组织中存在细胞胀亡；Ⅲ+Ⅳ临床分期胃癌组织中的胀亡指数（OI）高于Ⅰ+Ⅱ临床分期（P＜0.01）；淋巴结转移组OI明显高于无淋巴结转移组（P＜0.05）。AQP1表达强度与MVD呈显著正相关（P＜0.05）。在胃癌组织标本中，AQP1阳性表达率为58.2％，明显高于正常胃组织（P＜0.01）。胃癌伴淋巴结转移组AQP1表达水平明显高于无淋巴结转移组（P＜0.05）。多因素生存分析显示AQP1表达、有无淋巴结转移是预后的独立危险因素（P＜0.05）。结论细胞胀亡和AQP1表达与胃癌的血管生成、生物学行为密切相关；AQP1可以作为判断胃癌预后的临床指标。