[目的]分别以纯化的重组蛋白GST - MSA - 2c、GST - HSP20、GST - Tams1作为抗原,对2006～2008年全疆14个地州的牛梨形虫病流行病学进行调查.[方法]使用三种已建立的牛梨形虫病间接ELISA检测方法.[结果](1)在三年采集的1 340份血清样品中共检出牛梨形虫阳性血清280例,感染率为20.90％,其中牛巴贝斯虫、双芽巴贝斯虫、泰勒梨形虫单一感染率分别为8.21％ (23/280)、15.71％ (44/280)和43.93％ (123/280).(2)新疆牛梨形虫混合感染上升,为15.35％,甚至出现个别的三重感染.(3)2008年在流行区内的牛梨形虫感染率高达74％ (37/50),单一感染和混合感染分别64.85％ (24/37)和35.15％ (13/37),流行区的混合感染率明显增高.[结论]新疆牛梨形病的流行病学特征是感染率逐年增加,疫区不断扩大,混合感染使疫情趋于复杂化.这是新疆首次利用血清学方法对全疆牛梨形虫病进行大规模的流行病学调查.%[ Objective and Method ] The large scale of epidemiologic surveys for detection of bovine pi-roplasmosis in Xinjiang were performed by the improved indirect MSA - 2c ELISA, HSP20 ELISA Tamsl ELISA, respectively in this study. [ Result ] The results showed that (1) 280 positive serum examples were detected from 1,340 sera collected from 14 prefectures in Xijiang during the period from the year 2006 to 2008. The total infection rate reached 20.90% (280/1340). Among them, the infection rates of bovine piro-plasmosis caused by Babesia bovis, Babesia bigemina and Theileria annulata alon were 8. 92% (23/280), 15.71% (44/280) and 43.92% (123/280) respectively; (2) the rate of mix infections among them was rising, reaching 15.35% (43/280), including some triple infections; (3) The infection rate was unexpectedly increased to 74% (37/50) in some endemic areas where the disease occurred in 2008. The mixed infections reached 35.15% (13/50) in those areas. [Conclusion]The features of bovine Theileriosis in Xinjiang were that infection rate and mixed infections were increased year by year, which made epidemic situations more complicated. The situation was growing more and more dangerous for animals and human health. This was a first epidemiological survey on a large scale for detection of bovine piroplasmosis by three improved indirect ELISA assays using recombinant proteins as diagnostic antigens in Xinjiang.
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