南疆扁桃品种自交不亲和S-Rnase基因型鉴定

摘要

[Objective] The purpose of this program was to identify S - Rnase genotypes associated with self -incompatibility of almond cultivated in Xinjiang. [Method] 2 pairs of consensus primer PaCons II F + Pa Cons II - R and EM - PC2consFD + EM - PC3consRD which had been published for S - Rnase in prunus were used to amplify genome DNAs of the leaves from 10 almond cultivars. The specific fragments were cloned and sequenced, and then the nucleotide acid sequences were blasted on GenBank. [Result] The results showed: 10 Almond varieties were amplified and obtained bands with a total of 20, Searches in GenBank for homology with our sequences publicly available DNA databases revealed that 10 almond varieties contained 16 different S - Rnase genes, and 4 5 - Rnase gene fragments had been deposited in the GenBank as S1(1 370 bp) , 512(2 479 bp) , S14 (740 bp), 552(1 626 bp) and 12 S - Rnase gene fragments were novel 5 - Rnase genes, temporarily named as SA780 bp), SB(530 bp), Sc(l261 bp), SD(432 bp), SE(1266 bp), SF(270 bp), S(l263 bp), SH(272 bp),S1(690 bp),SJ(l 523 bp), 5K(755 bp), SL(l 486 bp). [Conclusion]Ten S - Rnase were identified in the research.%[目的]鉴定南疆扁桃品种自交不亲和S-RNase基因型,可为科学合理地配置授粉品种提供依据,有效提高南疆扁桃的产量.[方法]以南疆10个扁桃品种的叶片为试材,利用蔷薇科通用引物PaConsⅡ-F+PaConsⅡ-R、EM - PC2consFD+ EM - PC3consRD对叶片基因组DNA进行S-RNase特异性扩增,将克隆测序结果在GenBank上进行同源性比对.[结果]显示10个扁桃品种均获得2条带,共20条带,包含16种不同的S基因核苷酸序列,其中4个为GenBank上登录的已知S -RNase基因序列,分别为S1(1 370 bp)、S12(2 479bp)、S14(740bp)、S52(1 626bp),12个为新的S-RNase基因序列,暂时分别命名为SA(780bp)、SB(530bp)、SC(1261 bp)、SD(432 bp)、SE(1266bp)、5F(270 bp)、SG(1263 bp)、SH(272 bp)、SI(690 bp)、SJ(1 523bp)、SK(755bp)、SL(1 486bp).[结论]鉴定出10个品种的S- RNase基因型.