[目的]研究金钻蔓绿绒种苗扦插、播种、分株慢的生产技术,利用组织培养技术为金钻蔓绿绒规模化生产提供理论依据.[方法]以金钻蔓绿绒一年生茎段为试材,筛选其诱导、增殖的最佳培养基,简化生根、移栽方法,快速组培生产优质种苗为研究对象.[结果]金钻蔓绿绒的诱导培养基为MS+ BA1.5 mg/L+NAA0.1 mg/L,增殖培养基为MS+ BA0.2 mg/L+ NAA0.1 mg/L,气生根苗在炼苗成活率达98％,草炭和珍珠岩混合基质最适于移栽.[结论]建立金钻蔓绿绒的组培快繁体系,为金钻蔓绿绒优质种苗工厂化生产提供可靠的技术支撑.%[Objective] In order to solve the slow Philodendron con-go seedling cuttings,sowing,ramet production problems,this project aims to provide a theoretical basis for the large-scale production by taking advantage of tissue culture technology.[Method] The annual stems of Philodendron con-go were employed as experimental materials.The study assessed induction and proliferation medium,simplified rooting,transplanting method and rapid tissue culture for producing high quality seedlings.[Result]The results showed that the induction philodendron medium was MS plus BA1.5 mg/L with the addition of NAA0.1 mg/L,and the proliferation medium was MS plus BA 0.2 mg / L plus NAA0.1 mg / L,in which,the survival rate reached 98％.Peat and perlite mixed matrix was the most suitable for transplanting the aerial roots.[Conclusion] Technical research of tissue culture production technology of Philodendron con-go established rapid propagation system and provided reliable technical support for quality seedling factory production.
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