Expression of sphingosine kinase gene in the interactions between human gastric carcinoma cell and vascular endothelial cell

摘要

AIM: To study the interactions between human gastriccarcinoma cell (HGCC) and human vascular endothelialcell (HVEC), and if the expression of sphingosine kinase(SPK) gene was involved in these interactions.METHODS: The specific inhibitor to SPK, dimethylsphingosine (DMS), was added acting on HGCC andHVEC, then the cell proliferation was measured by MTT.The conditioned mediums (CMs) of HGCC and HVECwere prepared. The CM of one kind of cell was added tothe other kind of cell, and the cell proliferation wasmeasured by MTr. After the action of CM, the cellularexpression of SPK gene in mRNA level was detectedwith in situ hybridization(ISH).RESULTS: DMS could almost completely inhibit theproliferation of HGCC and HVEC. The growth inhibitoryrates could amount to 97.21%, 83.42 %, respectively(P＜0.01). The CM of HGCC could stimulate the growthof HVEC (2.70±0.0:1, P＜0.01) while the CM of HVEC couldinhibit the growth of HGCC (52.97±0.01 %, P＜0.01).There was no significant change in the mRNA level ofSPK gene in one kind of cell after the action of the CM ofthe other kind of cell.CONCLUSION: SPK plays a key role in regulating theproliferation of HGCC and HVEC. There exist complicatedinteractions between HGCC and HVEC. HGCC cansignificantly stimulate the growth of HVEC while HVECcan significantly inhibit the growth of HGCC. Theexpression of SPK gene is not involved in the interactions.