Protective effects of terminal ileostomy against bacterial translocation in a rat model of intestinal ischemia/reperfusion injury

摘要

AIM:To investigate the effects of terminal ileostomy on bacterial translocation(BT)and systemic inflammation after intestinal ischemia/reperfusion(I/R)injury in rats.METHODS:Thirty-two rats were assigned to either the sham-operated group,I/R group,I/R+resection and anastomosis group,or the I/R+ileostomy group.The superior mesenteric artery was occluded for 60 min.After 4 h,tissue samples were collected for analysis.BT was assessed by bacteriologic cultures,intestinal permeability and serum levels of endotoxin;systemic inflammation was assessed by serum levels of tumornecrosis factor(TNF)-α,interleukin(IL)-6 and IL-10,as well as by the activity of myeloperoxidase(MPO)and by intestinal histopathology.RESULTS:Intestinal I/R injury not only caused morphologic damage to ileal mucosa,but also induced BT,increased MPO activity and promoted the release of TNF-α,IL-6,and IL-10 in serum.BT and ileal mucosa injuries were significantly improved and levels of TNF-αand IL-6 in serum were decreased in the I/R+ileostomy group compared with the I/R+resection and anastomosis group.CONCLUSION:Terminal ileostomy can prevent the detrimental effects of intestinal I/R injury on BT,intestinal tissue,and inflammation.