Altered distribution of regulatory lymphocytes by oraladministration of soy-extracts exerts a hepatoprotectiveeffect alleviating immune mediated liver injury,non-alcoholic steatohepatitis and insulin resistance

摘要

AIM To determine the immune-modulatory and thehepatoprotective effects of oral administration of twosoy extracts in immune mediated liver injury and nonalcoholicsteatohepatitis （NASH）.METHODS： Two soy extracts, M1 and OS, were orallyadministered to mice with concanavalin A （ConA）immune-mediated hepatitis, to high-fat diet （HFD） miceand to methionine and choline reduced diet combinedwith HFD mice. Animals were followed for disease andimmune biomarkers.RESULTS： Oral administration of OS and M1 had anadditive effect in alleviating ConA hepatitis manifestedby a decrease in alanine aminotransferase and aspartateaminotransferase serum levels. Oral administration ofthe OS and M1 soy derived fractions, ameliorated liverinjury in the high fat diet model of NASH, manifested bya decrease in hepatic triglyceride levels, improvementin liver histology, decreased serum cholesterol andtriglycerides and improved insulin resistance. In themethionine and choline reduced diet combined withthe high fat diet model, we noted a decrease inhepatic triglycerides and improvement in blood glucoselevels and liver histology. The effects were associated with reduced serum tumor necrosis factor alpha andalteration of regulatory T cell distribution.CONCLUSION： Oral administration of the combinationof OS and M1 soy derived extracts exerted an adjuvanteffect in the gut-immune system, altering the distributionof regulatory T cells, and alleviating immune mediatedliver injury, hyperlipidemia and insulin resistance.