Inhibition of adenovirus-mediated p27kip1 gene on growth of esophageal carcinoma cell strain

摘要

AIM: To investigate the inhibition of p27kip1 gene on the growth of esophageal carcinoma cell strain (EC9706).METHODS: Recombinant adenovirus Ad-p27kip1 was constructed and transfected into esophageal carcinoma cell EC-9706, and its effect on p27kip1 expression, the growth of esophageal carcinoma cell, DNA replication, protein synthesis, cell multiplication and apoptosis were explored by means of cell growth count, 3H-TdR, 3H-Leucine incorporation,flow cytometry, DNA fragment analysis and TUNEL.RESULTS: Recombinant adenovirus Ad-p27kip1 was successfully constructed with a virus titer of 1.24x1012 pfu/ml.p27kip protein expression increased markedly after EC-9706 transfection, while incorporation quantity of 3H-TdR and 3HLeucine decreased significantly. The growth of esophageal carcinoma cell was inhibited obviously. Testing of flow cytometry displayed a typical apoptosis peak, and DNA gel electrophoresis showed a typical apoptosis ladder. TUNEL showed the apoptosis rate of Ad-p27kip1 group and control group to be 37.3 % and 1.26 % (P<0.001) respectively.CONCLUSION: Ad-p27kip1 can inhibit the growth and multiplication of esophageal carcinoma cells and induce apoptosis. Therefore, enhanced p27kip1 expression may be a new way to treat esophageal carcinoma.