EFFECTS OF ACUPUNCTURE ON INTRACELLULAR FREE CALCIUM AND MAGNESIUM CONCENTRATIONS IN CARDIAC MYOCYTES OF HEMORRHAGIC HYPOTENSION RABBITS

摘要

The effects of electroacupuncture on hypotension and the concentrations of intracellular free calcium([Ca2+]i) and free magnesium([ Mg2+]i) in cardiac myocyte of rabbit were studied. The hemorrhapic hypotension was induced by losing blood from femoral artery of rabbit. [Ca2+]i and [Mg2+]i were determined by use of AN-CM-COM cation measurment system and the Fura-2-AM and Furaptra-AM ionic probes. [Ca2+]i was significantly decreased, while [Mg2+]i was increased, and the ratio of [Ca2+]i / [ Mg2+]i was decreased as the blood pressure went down with losing blood. The blood pressure and [ Ca2+ ] i of treatment group rabbits were significantly raised by electroacupuncturing the points of "Renzhong "and "Chengjiang", and the ratio of [ Ca2+] i / [ Mg2+]i was increased. The [Ca2+]i and [ Mg2+]i in cardiac myocyte of normal rabbits had no clear changes by electroacupuncture. It suggested that intracellular free calcium and magnesium ion in cardiac myocyte may play an important role in regulating blood pressure by acupunoture.