延髓背柱核在穴位敏化现象中的作用

摘要

Objective:To explore the sensitization variance; of acupoint when internal organs is under pathological condition; to observe quantity- effect variance of dorsal column nuclei ( DCN ) to electro-acupuncture under both physiological and pathological condition; to explain the central mechanism of acupoint sensitization.Methods:A total of 23 adult male SD rats were used in this study.Visceral no-ciceptive stimulus was generated by colorectai distension ( CRD) and DCN Neuron activity was recorded.The quantity-effect variance of neuron activity to electro-acupuncture at Ziisanli-Shangjuxu area both before and after CRD was observed.Results:Visceral nocicep-tive afferent s could enhance neuron activity at DCN Neuron to acupoint acupuncture.DCN neuron activity caused by electro-acupuncture increased when visceral nociception rose.Conclusion ;The area of acupoint could be changed with visceral function.When the visceral function was damaged,the number of response points,area of acupoint receptive filed and sensitivity of acupoint would also change correspondingly.%目的:探讨内脏病理状态下穴位敏感程度的变化;观察电针刺激对延髓背柱核(DCN)神经元激活效应在正常和内脏伤害性传入情况下反应的量-效变化,阐述穴位敏化的中枢机制.方法:选用成年雄性SD大鼠,采用直结肠扩张(CRD)作为内脏伤害性刺激,记录延髓DCN神经元活动,观察不同强度CRD刺激前后电针刺激"足三里-上巨虚"穴区对DCN神经元的激活作用量-效反应的不同改变.结果:内脏的伤害性传入可以在DCN神经元观察到敏化穴位针刺的反应强度,并随着内脏伤害强度的增加,针刺对DCN神经元的激活作用也随之增加.结论:穴位可随内脏功能的变化而变化,当内脏功能受损时,体表相应反应点的数量、穴位感受野的大小和穴位的敏感程度也发生相应改变.