HPLC 法测定药用菊花中10个主要化学成分的含量

摘要

Objective:To establish an HPLC method for simultaneous determination of 10 contents including 5 caffeoylquinic acids and 5 flavones in Chrysanthemum Flower.Methods:HPLC separation was carried out with an DiamonsilTMC18 column(4.6 mm × 250 mm,5 μm)using acetonitrile-0.05% Formic acid as mobile phase at a flow rate of 1.0 mL/min.The detection wavelength was 335 nm and the column temperature was maintained at 40 ℃.Results:Good linear relationships were observed between the masses and the peak areas of the 10 contents;The average recovery rates were between 95.6 and 100.3%,RSD was between 0.66 and 1.95%.Conclusion:The content determination method can be used as an effective method for the quality control of Chrysanthe-mum Flower,which provides the data base for the research on the relationships between different contents of Chrysanthemum Flow-er.%目的：建立 HPLC 法测定菊花药材中10个主要化学成分的含量，包括5个咖啡酰奎宁酸类成分和5个黄酮类成分。方法：采用 DiamonsilTMC18（4．6 mm ×250 mm，5μm）色谱柱，流动相为乙腈-0．05％甲酸，流速1．0 mL／min，检测波长335 nm，柱温40℃。结果：10个化合物在一定范围内与峰面积呈良好的线性关系，r 值均大于0．9995；平均加样回收率（n ＝6）范围为95．6％～100．3％，RSD 为0．66％～1．95％。结论：本文所建立含量测定方法可作为中药菊花的质量控制的有效方法，为明确不同品种菊花的基原关系和品种鉴定研究提供了数据基础。