干寒属性食物与干寒环境因素对小鼠生物表征的影响

摘要

目的:观察干寒属性食物与干寒环境双因素对小鼠生物表征的影响.方法:将健康雄性ICR小鼠30只随机分为对照组和干预组各15只,每日称体质量、测进食量、饮水量、大便量、小便量并观察各种生物表征的变化.结果:生物表征方面,与对照组相比,干预组生物表征第14天表现出舌质暗红,少津,皮毛稀疏,少光泽,蜷缩少动,倦怠,淡漠,反应迟钝,扎堆眯眼,嗜睡,大便半成形、半湿,小便色清长;第21天表现出青紫舌,皮毛松弛,暗淡无光泽,蜷缩少动,倦怠淡漠,反应迟钝,扎堆眯眼,大便不成形、黏糊状,小便色清量少.体质量方面,与对照组相比,第7天干预组小鼠体质量减轻,但差异无统计学意义(P>0.05);第14天体质量增长缓慢,差异明显(P<0.05);第21天干预组较对照组体质量增长缓慢,具有显著性差异(P<0.01).出入量方面,与对照组相比,干预组小鼠饮水量明显增加,具有显著性差异(P<0.01);而饮食量、小便量,大便量2组比较差异无统计学意义(P>0.05).结论:干寒属性食物和干寒环境两种因素施加于小鼠后,可以使小鼠产生体质量减轻、饮水量增加等一系列生物表征改变,随着时间的延长或强度增加,干寒属性食物和干寒环境最终可能导致维吾尔医学干寒性气质失调证.%Objective:To observe the effects of the factors of dry cold food and dry cold environment on bio-logical characterization of the mice. Methods:Thirty healthy male ICR mice were randomized into the control group and the intervention group, 15 mice each group, body weight was weighed each day, food-intake, water consump-tion, stool volume and urine volume were detected, and the changes of biological characterization were observed.Results:The aspect of biological characterization, compared with the control group, biological characterization of the intervention group manifested dull-red tongue nature, less body fluid, sparse fur, less lustrous, crouching and moving less, lassitude, indifference, response lags, squint after flocking together, somnolence, semiformed stool, semiwet, clear urine in large amounts, manifested purple tongue, flabby fur, dim without luster, crouching and moving less, lassitude, indifference, response lags, squint after flocking together, deformed and sticky stool, clear urine in small amounts. The aspects of body weight, compared with the control group, body weight of the mice in the intervention group decreased at the seventh day, while the difference had no statistical meaning, body weight of the mice in-creased slowly at the 14th day, and the difference was obvious (P<0.05);body weight of the mice of the interven-tion group at the 21st day increased slowly compared with the control group, and there was a significant difference (P<0.01). Intake and output volume, compared with the control group, water consumption of the mice in the inter-vention group increased notably, and there was a significant difference (P<0.01);while there was no significant dif-ference(P>0.05) in the comparison of food intake volume, the volume of urine and stool. Conclusion:Dry cold food and dry cold environment could make the mice a series of changes of biological characterization, which contains body weight reduction, water consumption increase and others, as the time goes by and the intensity increases, dry cold food and dry cold environment could finally increase dry cold nature disorder pattern for uygur medicine.