假叶树组织培养植物激素应用筛选试验

摘要

Experiment of plant hormones on tender stem of Ruscus aculeatus in vitro was carried out. The screening results indi-cated that the highest induced rate of callus can be obtained in medium of MS+ZT 2.0 mg· L-1+IBA 0.5 mg· L-1 and the opti-mum medium is MS+ZT 1.5 mg · L- 1+IBA 0.2 mg · L- 1 for differentiation of adventitious bud. The growth coeffi-cient could be four to five times in medium of MS+ZT 1.0 mg· L-1-1.5 mg· L-1+IBA 0.2 mg· L-1 for multiplication culture. Root-ing of adventitious bud in medium of 1/2MS+NAA 1.0+1.5 g · L- 1 reached to 100 percent, the survival rate of seed-ling could reach to 95 percent or more after transplanting in mixture of perlite and peat soil in the ratio of 1 to 1.%采用假叶树的幼嫩茎段为外植体组织培养，对加入适合的植物激素作筛选试验，结果表明：MS+ ZT 2.0 mg·L-1+IBA 0.5 mg·L-1培养基愈伤组织的诱导率最高；MS+ZT 1.5 mg·L-1+IBA 0.2 mg·L-1培养基分化不定芽效果最佳，并且在MS+ZT 1.0 mg·L-1-1.5 mg·L-1+IBA 0.2 mg·L-1培养基上继代增殖培养增殖系数可达4～5倍；不定芽在1/2MS+NAA 1.0+1.5 g·L-1活性炭的培养基上，生根率达100%；生根小苗移栽珍珠岩与泥炭土（1∶1）基质上，成活率达95%以上。