银纳米颗粒的绿色合成:藻类生命周期对银纳米颗粒合成和长期稳定性的影响

摘要

Spherical Ag nanoparticles (AgNPs) were biologically synthesized using four different extracts prepared from Parachlorella kessleri algae cultivated for 1,2,3 and 4 weeks.The influence of algae life cycle on AgNPs formation and effect of different storage conditions on AgNPs long-term stability were investigated. The age of algae influenced the rate of AgNPs synthesis and amount of AgNPs in solution. The age of algae did not influence the AgNPs long-term stability. UV–vis and TEM observation revealed that long-term stability of AgNPs can be influenced by storage temperatures, and low temperature positively influences the AgNPs stability. AgNPs stored at dark and at temperature of ~5 ℃ showed the best long-term stability regardless of the culture age. Such AgNPs remained spherical, fine (5?20 nm) and stable (no agglomeration) even after 6 months.%用分别培养了1、2、3 和4周的凯氏拟小球藻的四种不同的提取物,生物合成球形银纳米颗粒(AgNPs).研究藻类生命周期对AgNPs合成的影响和不同的储存条件对AgNPs 长期稳定性的影响.结果发现,藻类的年龄会影响AgNPs 的合成率和溶液中的数量,但不影响AgNPs的长期稳定性.UV–vis 和 TEM 的结果显示,AgNPs的长期稳定性受储存温度的影响,低温更有利于其长期稳定性.储存在黑暗环境和5 ℃ 左右时,AgNPs 表现出最佳的长期稳定性,而与藻类年龄无关,存储6个月以后,这些AgNPs 仍然保持着球形,尺寸细小(5~20 nm),且无团聚.