In vitro evaluation of transdermal permeation effects of Fu's cupping therapy via six diffusion kinetics models

摘要

本研究建立吲哚美辛亲水凝胶贴剂的体外经皮渗透的6种动力学模型:零级、一级、Higuchi级、Ritger-Peppas级、Weibull级与Hixcon-Crowell级动力方程Q1/3=kt+b,评价付罐疗法(FCT)体外经皮促渗作用与特点.3％氮酮和5％氮酮化学促渗剂与离子导入为参比,用HPLC-工作曲线对吲哚美辛(IM)定量,进行体外透皮扩散,结果进行回归建模.结果表明:FCT促渗处理组能明显提高吲哚美辛的透皮效果,改善体外透皮的Higuchi级与Weibull级动力学参数,提高透皮速率与渗透系数,减低时滞.FCT作为新的物理经皮给药促渗新技术,效果明显,具有中医特色和重要的临床应用价值.%In this study, six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established, including zero-level, first-order, Higuchi-level, Ritger-Peppas, Weibull and Hixcon-Crowell dynamic equations. The chemical permeation enhancers, including 3％and 5％Azone, and iontophoresis were used as the control. Transdermal diffusion tests were performed in vitro and indomethacin was quantified by high performance liquid chromatography system. The transdermal parameter of the Higuchi and Weibull dynamic equations, indicated that Fu's cupping therapy (FCT) could significantly improve Higuchi and Weibull kinetic parameters in vitro transdermal, increased transdermal rate and permeability coefficient, reduced lagging time. Additionally, statistical analysis speculated the skin barrier function could be restored after 46 h treatment. Hence, as a new physical transdermal drug delivery technology, transdermal permeation effects produced by FCT are obvious, which has the characteristics of traditional Chinese medicine and has important clinical application value.